International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. HucMSC-ex promoted fibroblast-to-myofibroblast differentiation in inflammatory environments. Cardiac fibroblasts were treated with LPS (100 ng/ml) or LPS+hucMSC-ex (200 μg/ml) for 24 h; the control group did not receive any treatment. (A, B) Colla-gen I, Periostin, and α-SMA expre-ssion were detected by western blot. (C) Collagen contraction analysis was used to determine the collagen contractility of cardiac fibroblasts treated with LPS (100 ng/ml) or LPS+hucMSC-ex (200 μg/ml) for 24 h. (D) Cardiac fibroblasts were treated with LPS (100 ng/ml) or LPS+hucMSC-ex (200 μg/ml) for 24 h, Transwell migration assays were used to detect the migration ability of cardiac fibro-blasts. Scale bar=100 μm; *p< 0.05, **p<0.01, and ***p<0.001.
International Journal of Stem Cells 2021;14:320-30 https://doi.org/10.15283/ijsc20186
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