International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. During osteogenic differentiation of BMSCs, miR-34c-5p expression, ALP content and calcium deposition were promoted. (A) Abnormal expressions of miRNAs during osteogenic differentiation of mesenchymal stem cells obtained from the GEO database (GSE115197 and GSE19232). (B) The expressions of miR-34c-5p, miR-199a-5p, miR-324-5p and miR-361-5p in rabbit lumbar fusion model rabbit or normal rabbit were determined by qRT-PCR. (C) qRT-PCR was used to detect the expression of miR-34c-5p in BMSCs with different differentiation times (0, 3, 7 and 14 day). (D) ELISA was used to measure the content of ALP in BMSCs at different differentiation times (0, 3, 7 and 14 day). (E) Alizarin red staining of BMSCs at different differentiation times (0, 3, 7 and 14 day). Scales: 200 μm; magnifications: ×200. ^vs. Control, *vs. 0 days. ^^^ or ***p<0.001. BMSCs, bone marrow mesenchymal stem cells; ALP, alkaline phosphatase; GEO, gene expression omnibus; qRT-PCR, quantitative reverse transcription polymerase chain reaction; ELISA, enzyme linked immune sorbent assay. The experiment was independently repeated three times.
International Journal of Stem Cells 2021;14:286-97 https://doi.org/10.15283/ijsc20188
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