International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. miR-34c-5p decreased cell proliferation and increased ALP content and calcium deposits of BMSCs. (A) The expression of miR-34c-5p in BMSCs transfected with miR-34c-5p mimic or mimic control was detected by qRT-PCR. (B) The expression of miR-34c-5p in BMSCs transfected with miR-34c-5p inhibitor or inhibitor control was detected by qRT-PCR. (C) The cell proliferation of BMSCs transfected with miR-34c-5p mimic or mimic control was identified by CCK-8 at different times (1, 3, 5 and 7 day). (D) The cell proliferation of BMSCs transfected with miR-34c-5p inhibitor or inhibitor control was identified by CCK-8 at different times (1, 3, 5 and 7 day). (E) The ALP content of BMSCs transfected with miR-34c-5p mimic or mimic control was detected by ELISA. (F) The ALP content of BMSCs transfected with miR-34c-5p inhibitor or inhibitor control was detected by ELISA. (G) Alizarin red staining of BMSCs transfected with miR-34c-5p mimic or mimic control at 14 days. Scales: 200 μm; magnifications: ×200. (H) Alizarin red staining of BMSCs transfected with miR-34c-5p inhibitor or inhibitor control at 14 days. Scales: 200 μm; magnifications: ×200. *vs. MC; ^vs. IC. * or ^p<0.05; ** or ^^p<0.01; *** or ^^^p<0.001. BMSCs, bone marrow mesenchymal stem cells; qRT-PCR, quantitative reverse transcription polymerase chain reaction; CCK-8, cell counting 8; ALP, alkaline phosphatase; ELISA, enzyme linked immune sorbent assay; I, miR-34c-5p inhibitor; IC, inhibitor control; M, miR-34c-5p mimic; MC, mimic control. The experiment was independently repeated three times.
International Journal of Stem Cells 2021;14:286-97 https://doi.org/10.15283/ijsc20188
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