International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. MiR-34c-5p promoted calcium deposits and the expression of RUNX2 and OCN of BMSCs by inhibiting Bcl-2 expression. (A) Alizarin red staining of BMSCs treated or untreated with miR-34c-5p mimic and/or Bcl-2 at 14 days. Scales: 200 μm; magnifications: ×200. (B) Alizarin red staining of BMSCs treated or untreated with miR-34c-5p inhibitor and/or siBcl-2 at 14 days. Scales: 200 μm; magnifications: ×200. (C, D) The expression of Bcl-2, RUNX2 and OCN of BMSCs treated or untreated with miR-34c-5p mimic and/or Bcl-2 were detected by Western blot. (E) The expressions of Bcl-2, RUNX2 and OCN of BMSCs treated or untreated with miR-34c-5p mimic and/or Bcl-2 were detected by qRT-PCR. (F) The expressions of Bcl-2, RUNX2 and OCN of BMSCs treated or untreated with miR-34c-5p inhibitor and/or siBcl-2 were detected by qRT-PCR. (G, H) The expressions of Bcl-2, RUNX2 and OCN of BMSCs treated or untreated with miR-34c-5p inhibitor and/or siBcl-2 were detected by Western blot. *vs. MC, #vs. M, vs. BCL2, ^vs. IC, §vs. I, vs. siBCL2. or * or p<0.05; ^^ or ††p<0.01; *** or ^^^ or ‡‡‡ or ### or §§§p<0.001. BMSCs, bone marrow mesenchymal stem cells; CCK-8, cell counting 8; ALP, alkaline phosphatase; ELISA, enzyme linked immune sorbent assay; Bcl-2, B cell lymphoma/lewkmia-2; I, miR-34c-5p inhibitor; IC, inhibitor control; M, miR-34c-5p mimic; MC, mimic control. The experiment was independently repeated three times.
International Journal of Stem Cells 2021;14:286-97 https://doi.org/10.15283/ijsc20188
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