International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. S6K1 was downstream target of miR-181a in cells. (A) Schematic diagram of a conserved putative miR-181a targeting site in 3’UTR of S6K1 (WT). The site mutations were introduced to miR-181a targeting site of 3’UTR (MUT). (B) Luciferase reporter assay study of the interaction between miR-181a and 3’UTR of S6K1. GC1 spg cells were transfected with luciferase reporter genes conjugated with either wildtype (WT) or mutant (MUT) 3’UTR of S6K1. 24 hours later, the cells were transfected further with either miR-181a mimic or scramble control. Transfected cells were cultured for 48 hour and then harvested for the measurement of luciferase activities in a plate reader. (C) Western blotting analysis of mTOR1, S6K1, p-S6K1 in cells transfected with either miR-181a or scramble control. The representative image from at least three independent experiments was shown. The representative images from at least three independent experiments were shown. The data were presented as means±SD (*p<0.05, **p<0.01, ***p< 0.001 as compared with the control).
International Journal of Stem Cells 2021;14:341-50 https://doi.org/10.15283/ijsc21001
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