International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

Download original image
Fig. 5. Inhibiting S6K1 activity rescued the antagonizing effects of miR-181a inhibitor in GC-1 spg cell growth. (A) Western blotting analysis of S6K1 and p-S6K1 in GC1 spg cells transfected with control or miR-181a inhibitor. (B) Cell proliferation analysis of GC-1 spg cells transfected with control or miR-181a inhibitor. In parallel, the miR-181a inhibitor transfected cells were also treated with S6K1 kinase inhibitor or DMSO. The growth rate of cells was monitored using MTT kit for 72 h. (C) Representative histogram data of the cell-cycle analysis of GC-1 spg cells in different treatment conditions as described above. The representative images from at least three independent experiments were shown. The data were presented as means±SD (*p<0.05, **p<0.01, ***p<0.001 as compared with control).
International Journal of Stem Cells 2021;14:341-50 https://doi.org/10.15283/ijsc21001
© 2021 International Journal of Stem Cells