International Journal of Stem Cells : eISSN 2005-5447

Table. 1.

Table. 1.

MSC-derived exosomes in preclinical models of ARDS and ALI

PubMed EVs-MSCs source Disease model Therapeutic EVs dose Outcomes
23939814 Human bone marrow Male C57BL/6 mice with E. coli endotoxin-induced ALI EVs from 750,000 cells

Reduced inflammatory cell influx

Reduced EVLW

Reduced alveolar MIP-2 protein

Expression of KGF mRNA

Increased KGF

Increased IL-10

30682335 Human bone marrow Male C57BL/6 mice with lung ischemia/reperfusion EVs from 2×106 cells

Reduced lung edema

Reduced IL-8, IL-6, IL-17, IL-1β, TNF-α, HMGB1

Reduced alveolar macrophage M1 polarization

Murine primary pulmonary endothelial cells
30076187 Human bone marrow Mice with E. Coli induced ALI EVs from 1×106 cells

Increased AFC rate

Increased antimicrobial effect

Decreased lung protein permeability

Decreased bacterial CFU

25847030 Human bone marrow Ex vivo perfused human lung (rejected for the transplant) 100 μl EVs

Increased AFC rate

Decreased lung weight gain

Increased lung compliance

Decreased pulmonary artery pressure and resistance

Increased NO in perfusate

Decreased pH of perfusate

Decreased elevation of lactate

28376568 Human bone marrow P. aeruginosa induced mouse 10 μl EVs from 1×106 cells

Decreased WBC influx

Decreased MIP-2 secretion

Restoration of pulmonary capillary

Expression of Ang-1 mRNA

Increased alveolar Ang-1

Decreased TNF-α

Increased IL-10

26067592 Human bone marrow Male C57BL/6 mice with E. coli induced ALI 30 or 60 μl (intratracheal)
90 μl (i.v. injection)

Decreased total bacterial load

Decreased inflammation

Decreased lung protein permeability

Increased monocyte phagocytosis

Decreased TNF-α by LPS primed human monocytes

Increased COX2 and IL-10 mRNA expression

Increased IL-10 secretion by monocytes

Decreased bacterial CFU

29378639 Human bone marrow Influenza virus-infected pig EVs from 10×106 cells

Decreased Haemagglutination activity of influenza viruses

Decreased virus replication

Decreased lung inflammation

Decreased virus replication

Decreased pro-inflammatory cytokine production

Increased IL-10

29737632 Human bone marrow HLMVECs injured by cytomix (IL-1β, TNF-α, and IFN-γ) 20 μl EVs from 1×106 cells

Restoring protein permeability across injured HLMVECs

Preventing actin stress fibers formation

Preventing reorganization of cytoskeleton protein F-actin into actin stress fiber in cytomix injured HLMVEC

Restoring the VE-cadherin (adherens junction) and ZO-1 (tight junction) in HMVEC injured by cytomix

Increased Ang-1 mRNA expression

30870158 Human umbilical cord Rat with E. coli induced ALI EVs from 100×106 cells

Increased survival

Reduced alveolar-arterial oxygen gradient

Reduced alveolar protein leak

Increased lung mononuclear phagocytes

Reduced alveolar TNF-α concentrations

Enhanced endothelial NOS production

Abbreviations: EVs: Extracellular Vesicles, HLMVECs: Human Lung Micro Vascular Endothelial Cells, ZO: Zonula Occludens, MSC: Mesenchymal Stem Cells, EVLW: Extravascular Lung Water, MIP-2: Macrophage Inflammatory Protein 2, KGF: Keratinocyte Growth Factor, IL: Interleukin, TNF: Tumor Necrosis Factor, HMGB 1: High Mobility Group Box 1, CFU: Colony-Forming Unit, WBC: White Blood Cell, LPS: Lipopolysaccharides, COX: Cyclooxygenase, NOS: nitric oxide synthase.

International Journal of Stem Cells 2021;14:252-61 https://doi.org/10.15283/ijsc20182
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