International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. miR-324-5p targets TOLLIP expression in myocardial tissues of MI rats. Note: A, downstream target gene of miR-324-5p predicted by miRDB (http://mirdb.org/index.html) and Targetscan (http://www.targetscan. org/vert_71/) databases. B, binding site between miR-324-5p and TOLLIP was predicted by Targetscan data-base. C, binding relation of miR-324-5p and TOLLIP was verified through dual-luciferase reporter gene assay. D and E, expressions of miR-324-5p (D) and TOLLIP (E) were measured by RT-qPCR. N=6, the cell experiment was repeated three times; the results in panel C were shown as mean±standard deviation. Two-way ANOVA was used to analyze data in panel C, and Sidak’s multiple comparisons test was applied for post hoc test. One-way ANOVA was used to analyze data in panels D and E, and Tukey’s multiple comparisons test was applied for post hoc test. **p<0.01. MI: myocardial infarction, NC-ADSCs: ADSCs after lentivirus infection with miR-324-5p negative control, miR-ADSCs: ADSCs after lentivirus infection with miR-324-5p overexpression vectors.
International Journal of Stem Cells 2021;14:298-309 https://doi.org/10.15283/ijsc21025
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