International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

Download original image
Fig. 2. Identification of the hiPSC derived cardiomyocytes. (A) Schematic illustration of an experimental protocol for the cardiomyocytes differentiation from hiPSC via sequential transient modulation of canonical Wnt signaling pathway using GiWi method. Gi, GSK3 inhibitor; Wi, Wnt inhibitor; iCM, induced cardiomyocytes; IF, immunofluorescence; FC, flow cytometry. (B) Time course analysis of the expression of cardiac progenitor marker (NKX2-5) and cardiomyocytes marker (TNNT2) was performed using qRT-PCR assay. (C) The protein of cardiac progenitor marker (NKX2-5) and cardiomyo-cytes marker (cTNT and α-ACTININ) was further evaluated by IF method. Data are presented as mean±SD.
International Journal of Stem Cells 2021;14:410-22 https://doi.org/10.15283/ijsc21051
© 2021 International Journal of Stem Cells