International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Knockdown of P53 gene decreases the cardiomyocytes generation from hiPSC. (A) The qRT-PCR results showing successful knockdown of P53 in HiPSC. (B) Representative image of Western Blot displayed significantly decreased p53 protein level of lenti-Sh-P53 group (B) and quantification was done by densitometry (C). (D, E) Knockdown of P53 significantly declined mRNA levels of makers of cardiomyocytes (TNNT2) (D) and cardiac progenitor (NKX2-5) (E). (F, G) Representative image of flow cytometry (F) displayed notably decreased cTNT cardiomyocytes differentiated by hiPSC by knockdown of P53 in contrast with control,and quantification was done (G). (H, I) The qRT-PCR results showed that knockdown of P53 markedly reduced the mRNA levels of mesendodermal markers (BRACHYURY and EOMES) at day 3 from differentiation (E) and cardiac mesodermal makers (MESP1 and KDR) at day 5 (F), respectively. Expression values of the PCR analysis were normalized to GAPDH. Data are presented as mean±SD (t test, *p<0.05; n=3, performed in 3 independent experiments).
International Journal of Stem Cells 2021;14:410-22
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