International Journal of Stem Cells : eISSN 2005-5447

Fig. 7.

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Fig. 7. Upregulated WNT3 by p53 could further amplify the activation of Wnt signaling pathway caused by GSK inhibition (Gi). However, the amplified Wnt pathway by p53 was completely blocked by Wnt inhibitor (Wi) at late stage. (A, B) The Western Blot result showing at day 1 from differentiation, overexpressing P53 or WNT3 alone could increase the localization of β-catenin in the nuclear fraction indicating activation of Wnt pathway. Moreover, P53 overexpression together with GSK inhibitor (Gi) could further amplify the activation of Gi-caused activation of Wnt signaling as shown by the representative image of Western Blot (A) and quantification by densitometry (B). (C, D) The representative image of Western Blot showed that synergically increased Wnt signaling could remarkably abrogated by WNT inhibitor (Wi) at day 5, indicated by significantly decreased nuclear β-catenin level (C) and quantification was done by densitometry (D). Expression values of the Western Blot analysis of nuclear and total protein were normalized to Histone H3 and GAPDH respectively. Data are presented as mean±SD (t test, *p<0.05, **p<0.01; n=3, performed in 3 independent experiments).
International Journal of Stem Cells 2021;14:410-22 https://doi.org/10.15283/ijsc21051
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