International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. MiR-148a-3p mimic inhibited the cell viability, invasion, and differentiation of hDPSCs by down-regulating the expressions of DSPP, DMP-1, RUNX2, OCN, and Smad4. (A) The transfection efficiency of miR-148a-3p mimic was detected by RT-qPCR. U6 was used as an internal control. (B) The cell viability of hDPSCs was detected by MTT assays. (C, E) The invasion of hDPSCs was detected by Transwell assays. (D) The formation of calcium nodules in hDPSCs after being cultured for 14 days was detected by Alizarin red staining. (F) The expressions of DSPP, DMP-1, RUNX2, OCN, and Smad4 in hDPSCs were detected by RT-qPCR. GAPDH was used as an internal control. (G, H) The protein expressions of DSPP, DMP-1, RUNX2, OCN, and Smad4 in hDPSCs were detected by Western blot. GAPDH was used as an internal control. All experiments were conducted in triplicate (*p< 0.05, **p<0.01, ***p<0.001, vs. MC) (hDPSCs: human dental pulp stem cells, MC: mimic control, M: miR-148a-3p mimic).
International Journal of Stem Cells 2021;14:434-46 https://doi.org/10.15283/ijsc20118
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