International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. MiR-148a-3p reversed the regulatory effect of Wnt1 on the cell viability, invasion, and differentiation of hDPSCs. (A) Infection efficiency of Wnt1 and siWnt1 was detected by RT-qPCR. GAPDH was used as an internal control. (B) Transfection efficiency of miR-148a-3p inhibitor was detected by RT-qPCR. U6 was used as an internal control. (C, D) The cell viability of hDPSCs was detected by MTT assays. (E∼H) The invasion of hDPSCs was detected by Transwell assays. (I, J) The formation of calcium nodules in hDPSCs was detected by Alizarin red staining. All experiments were conducted in triplicate (*p<0.05, **p<0.01, ***p< 0.001, vs. NC; ^p<0.05, ^^p<0.01, vs. siNC; #p<0.05, ##p<0.01, ###p<0.001, vs. MC; p<0.05, △△p<0.01, vs. IC; p< 0.05, ++p<0.01, +++p<0.001, vs. M; &p< 0.05, &&p<0.01, vs. Wnt1; §p<0.05, §§p< 0.01, vs. I; p<0.05, ‡‡p<0.01, vs. siWnt1) (hDPSCs: human dental pulp stem cells, NC: negative control, siNC: small interfering RNA for negative control, MC: mimic control, IC: inhibitor control, M: miR-148a-3p mimic, I: miR-148a-3p inhibitor).
International Journal of Stem Cells 2021;14:434-46 https://doi.org/10.15283/ijsc20118
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