International Journal of Stem Cells : eISSN 2005-5447

Fig. 6.

Download original image
Fig. 6. MiR-148a-3p reversed the regulatory effect of Wnt1 on the activation of Wnt1/β-catenin and the expressions of DSPP, DMP-1, RUNX2, OCN, and Smad4. (A, B) The expressions of Wnt1, β-ca-tenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 in hDPSCs were detected by RT-qPCR. GAPDH was used as an internal control. (C∼F) The protein expressions of Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 in hDPSCs were detected by Western blot. GAPDH was used as an internal control. All experiments were conducted in triplicate (***p<0.001, vs. NC; ^^^p< 0.001, vs. siNC; ###p<0.001, vs. MC; △△△p<0.001, vs. IC; +++p<0.001, vs. M; &&&p<0.001, vs. Wnt1; §§§p< 0.001, vs. I; ‡‡‡p<0.001, vs. siWnt1) (hDPSCs: human dental pulp stem cells, NC: negative control, siNC: small interfering RNA for negative control, MC: mimic control, IC: inhibitor control, M: miR-148a-3p mimic, I: miR-148a-3p inhibitor).
International Journal of Stem Cells 2021;14:434-46 https://doi.org/10.15283/ijsc20118
© 2021 International Journal of Stem Cells