International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. Irisin promotes the paracrine efficacy of BMSCs through the PI3K/AKT pathway. (a) Conditioned media of equivalent cell numbers (1×106 cells) were collected for irisin-treated BMSCs and control groups. By employing HUVECs, tube forma-tion was assessed using the supernatant of BMSCs treated with irisin and control, irisin alone, and control alone. Scale bar, 50 μm. (b) The wound-healing assay was performed to demonstrate HUVEC migration using the above conditioned media, respectively. Scale bar, 243.2 μm. (c) The number of branch points was quantified by Image-Pro software and is shown in bar graphs (n=3 separate studies). (d) Quantification of HUVEC migration by Image-Pro software (n=3). (e) Western blot analyses of pPI3K, PI3K, p-AKT, AKT, p-ERK1/2, ERK1/2, p-P38, P38, p-JNK, and JNK pathway were detected in irisin-treated BMSCs and control groups. All data were measured as mean±standard error of the mean (SEM). *p<0.05. BMSCs, bone marrow mesenchymal stem cells; HUVECs, human umbilical vein endothelial cells.
International Journal of Stem Cells 2021;14:455-64 https://doi.org/10.15283/ijsc21005
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