International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. The PI3K/AKT pathway is required to mediate the paracrine efficacy of BMSCs. (a) Representative images of tube formation were analyzed using HUVECs co-cultured with the supernatant of BMSCs treated with irisin, LY294002, irisin´╝őLY294002, or control, respectively. Scale bar, 50 μm. (b) The number of branch points in the above groups was quantified by Image-Pro software and is shown in bar graphs (n=3). (c) The wound-healing assay assessed HUVEC migration using the above conditioned media, respec-tively. Scale bar, 243.2 μm. (d) Quantification of HUVEC migration (n=3) by Image-Pro software. Data are presented as mean±standard error of the mean (SEM). *p<0.05. BMSCs, bone marrow mesenchymal stem cells; HUVECs, human umbilical vein endothelial cells.
International Journal of Stem Cells 2021;14:455-64 https://doi.org/10.15283/ijsc21005
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