International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. miR-18a-5p and HIF-1α are downstream targets of circ-Gcap14. (A) Predicted binding sites for miR-18a-5p in circ-Gcap14. The mutated circ-Gcap14 is also shown. (B) Relative luciferase activity at 48 h post-transfection of HEK293T cells with miR-18a-5p mimics/NC or circ-Gcap14 wild-type/mutant. Data are presented as the mean±SD. ***p<0.001. (C) Binding site prediction of miR-18a-5p in the HIF-1α 3’UTR. The mutant version of the 3’-UTR-HIF-1α is shown. (D) Relative luciferase activity at 48 h post-transfection of HEK293T cells with miR-18a-5p mimic/NC or 3’UTR-HIF-1α wild-type/mutant. Data are presented as the mean±SD. ***p<0.001. (E∼G) RT-qPCR shows circ-Gcap14 (E), miR-18a-5p (F) and HIF-1α expression (G). Data are presented as the mean±SD. ***p<0.001 vs. NC group. ###p<0.001 vs. circ-Gcap14.
International Journal of Stem Cells 2021;14:447-54 https://doi.org/10.15283/ijsc21050
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