International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. Process of sheep induced pluripotent stem cell (siPSC) generation and reprogramming efficiency calculation of siPSC. (A) Schematic diagram of sheep kidney cells (SKCs) reprogramming protocol used. (B) AP staining of siPSC generation with 8 factors (Oct4/Sox2/Klf4/c-Myc/Nanog/Lin28/hTERT/SV40LT). (C) AP staining of siPSC generation with 8 factors plus miR200c-141. (D) Magnification image of representative AP-positive siPSC colonies (100×). (E) The number of AP-positive colonies was counted to calculate the reprogramming efficiency. Data presented as the mean±standard deviation (SD), n=3, **p<0.01.
International Journal of Stem Cells 2021;14:423-33 https://doi.org/10.15283/ijsc21080
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