International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Oar-miR-200c hinders ZEB1 expression and upregulates E-cadherin expression during SKCs repro-gramming. (A) Schematic representation of ZEB1 3’UTR cloned into luciferase reporter vector-pmiRGLO. The 6 binding sites sequence in the 3’UTR and the corresponding mutations for miR-200c are underlined. (B) miR-200c directly targeted the ZEB1 3’UTR by dual-luciferase reporter assay. (C) Overexpressing miR-200c in SKCs down-regulated of ZEB1 and up-re-gulated of E-cadherin transcription levels by qRT-PCR. (D) Overex-pressing miR-200c in SKCs down-regulated of ZEB1 and up-regulated of E-cadherin protein expression by western blotting. β-actin is the loading control. Data presented as the mean±SD, n=3, *p<0.05, **p< 0.01. ZEB1: zinc finger E-box-binding homeobox 1.
International Journal of Stem Cells 2021;14:423-33 https://doi.org/10.15283/ijsc21080
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