International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. Identifying induction factors for PSCs-derived EPCs. (A) A schematic diagram of inducing human PSCs-derived ECs. (B) The impact of cell seeding number on differentia-tion efficiency of PSCs-derived EC was analysed by flow cytometry. (C) In the second step of PSCs-derived EC differentiation, flow cytometry analysis showed adding BMP4 promoted PSCs-derived EC differentia-tion efficiency. (D, E) In the second step of EC differentiation, increasing SB431542 dose (5∼20 μM) promoted PSCs-derived EC differentia-tion efficiency. (F, G) The inhibitor Y27632 enhanced 202-iPSCs-deri-ved EC differentiation efficiency in the first step of differentiation in which Y27632 was used for three days. Statistics of CD31+CD34+ cells, CD31+ cells, CD34+ cells and CD43+ cells. Data are represented as the mean±SD. n=3∼4, the experiments were repeated more than three independent times. Student’s t test was performed (*p<0.05, **p< 0.01, ***p<0.001).
International Journal of Stem Cells 2022;15:122-35 https://doi.org/10.15283/ijsc21044
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