International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. DMSO improves EC and HE cell differentiation efficiency. (A, B) PSCs-derived EC (H1EC) differentia-tion efficiency increased when the cells were treated with DMSO in the first step for one day or three days. Statistics of CD31+CD34+ cells, CD31+ cells, CD34+ cells and CD43+ cells were analysed by flow cytometry. (C, D) Improved protocol for the HE differentiation of PSCs. A schematic digram of the stepwise induction process was shown. DMSO treatment for three days in the first step promoted the differentiation efficiency as analysed by flow cyto-metry. Statistics of APLNR+ cells, CD31+CD34+ cells, CD31+ cells, CD34+ cells and CD43+cells. Data were represented as the mean±SD, n=3∼4, and the experiments were repeated more than three times. Student’s t test was performed (ns: not significant, **p<0.01,****p< 0.0001).
International Journal of Stem Cells 2022;15:122-35 https://doi.org/10.15283/ijsc21044
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