Fig. 5. PSCs-derived ECs have characteristics of EPCs. (A) Comparison of the cell morphology of PSCs-derived ECs (H7EC, 202EC) with peripheral blood-derived normal EPCs (normal EPC1, normal EPC2) and IPAH-EPCs (IPAH EPC1, IPAH EPC2) (scale bars: 50 μm). (B) qRT-PCR of NRP1, which was reported to promote ECFC proliferation. GAPDH was used as an internal control. (C) ECs from DAY 5 or DAY 7 were analysed by the expression of the genes CD133, EFNB2, and EPHB4. GAPDH was used as an internal control. Data were represented as the mean±SD, n=3, and the experiments were repeated three times and analysed with student’s t test (*p<0.05, **p< 0.01, ***p<0.001).

International Journal of Stem Cells 2022;15:122-35 https://doi.org/10.15283/ijsc21044

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