International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

Download original image
Fig. 1. Alternative splicing of RUNX2 mRNA in dental pulp stem cells (DPSC). (A) Schematic diagram of isoforms of human RUNX2. Exon 5 and 7 are two alternative exons. (B) Analysis of alternative splicing of RUNX2 mRNA in DPSCs by RT-PCR. GAPDH served as a loading control. Diagrams on the left and right show the structure and size of RUNX2 pre-mRNA and spliced pro-ducts, and the location of primers (short lines above or below exons). The sizes of marker bands from the top to the bottom are 700 bp, 500 bp, 400 bp, 300 bp, and 200 bp, respectively. (C) Flow cytometric analysis of the purity of cultured dental pulp stem cells with stem cell biomarkers. CD90, CD29 and CD146 are mesenchymal stem cell markers, whereas CD34 and CD45 are hematopoietic stem cell markers.
International Journal of Stem Cells 2022;15:301-10 https://doi.org/10.15283/ijsc21035
© 2021 International Journal of Stem Cells