International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

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Fig. 3. RUNX2 exon 5 inclusion and YBX1 expression are enhanced after mineralization induction. (A) Alizarin red staining of DPSCs treated with or without mineralization induction for 21 days. (B, C) OC and DSPP expression (B), and alternative splicing of RUNX2 exon 5 in DPSCs after three days of mineralization induc-tion (C) were analyzed by RT-PCR. GAPDH served as loading control. (D) Summary of four independent experiments for the ratios of inclusion versus exclusion of RUNX2 exon 5 in DPSCs after mineralization induction. L and S represent products with or without exon 5. (E) YBX1 protein expression increased after three days of mineralization induction in DPSCs. (F) Histogram showed quantification of three independent experiments for the relative levels of YBX1. Actin served as loading control. Statistical comparisons of means were performed with Student’s t-test.
International Journal of Stem Cells 2022;15:301-10 https://doi.org/10.15283/ijsc21035
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