International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. YBX1 promotes RUNX2 exon 5 inclusion in DPSCs. (A∼C) Overexpression of YBX1 promoted RUNX2 exon 5 inclusion in HEK 293 cells. (D∼F) Overexpression of YBX1 promoted RUNX2 exon 5 inclusion in DPSCs. (B, E) Overexpression of Myc-tagged YBX1 was confirmed by Western blot. (C, F) Summary of three independent experiments for the ratios of inclusion versus exclusion of RUNX2 exon 5 in HEK 293 (C) or DPSCs (F). (G) Knockdown of YBX1 reduced RUNX2 exon 5 inclusion in DPSCs. (H) Histogram showed quantification of three independent experiments for the ratios of inclusion versus exclusion of RUNX2 exon 5 in DPSCs. (I) Knockdown efficiency of YBX1 and the expression of RUNX2 were analyzed by Western blot. GAPDH served as loading control. (J) Histogram showed quantification of three independent experiments for the expression of RUNX2 protein in DPSCs.
International Journal of Stem Cells 2022;15:301-10 https://doi.org/10.15283/ijsc21035
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