International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. In vivo osteogenic potential of BMSCs. In vivo transplantation assays were performed by combining hMSCs with HA/TCP followed by subcutaneous transplantation into immunocompromised mice. (A, B) H&E staining. (A) Implants from hMSC-HD cultures and (B) hMSC-AML cul-tures. hMSCs-HD formed ectopic ossicles that were sometimes populated by host haematopoietic marrow (aste-risk). The arrowheads indicate osteo-cytes. (C∼H) Micro-CT analysis. (C) Bone tissue formed from hMSCs-HD and (D) hMSCs-AML from the 3D reconstruction of implants. For better visualization of the bone tissue for-med (red), part of the HA/TCP (grey) was removed. (H) Tissue mineral density of implants formed from hMSCs-HD and hMSCs-AML. (E) Analysis of bone volume, (F) the relationship between bone volume and tissue volume, (G) and bone tissue thickness in implants formed from hMSCs-HD and hMSCs-AML. (I∼L) Human origin of the woven bone by immu-nohistochemical analysis. Expression of BMP4 within the woven bone from (I) hMSCs-HD and (J) hMSCs-AML. Expression of Osterix within the woven bone from (K) hMSCs-HD and (L) hMSCs-AML. HA/TCP=hydroxyapatite/tricalcium phosphate.
International Journal of Stem Cells 2022;15:227-32 https://doi.org/10.15283/ijsc21138
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