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Primers used for RT-PCR of pluripotent and CM markers
| Description | Gene name | REPSEQ_ID | Sequence | Product size (bp) | |
|---|---|---|---|---|---|
| Pluripotency marker | NM_203289.5 | F | ACCCCTGGTGCCGTGAA | 190 | |
| R | GGCTGAATACCTTCCCAAATA | ||||
| NM_024865.2 | F | AAAGGCAAACAACCCACT | 270 | ||
| R | GCTATTCTTCGGCCAGTT | ||||
| NM_003106.3 | F | GGGAAATGGGAGGGGTGCAAAAGAGG | 151 | ||
| R | TTGCGTGAGTGTGGATGGGATTGGTG | ||||
| NM_003212.3 | F | TCCTTCTACGGACGGAACTG | 140 | ||
| R | AGAAATGCCTGAGGAAAGCA | ||||
| Cardiogenic marker | NM_002202.2 | F | GAGGGTTTCTCCGGATTTGG | 101 | |
| R | TCCCATCCCTAACAAAGCATGT | ||||
| NM_004387.3 | F | TTCCCGCCGCCCCCGCCTTCTAT | 139 | ||
| R | CGCTCCGCGTTGTCCGCCTCTGT | ||||
| NM_001001430.2 | F | TTCACCAAAGATCTGCTCCTCGCT | 166 | ||
| R | TTATTACTGGTGTGGAGTGGGTGTGG | ||||
| NM_002471.3 | F | TCTCCGACAACGCCTATCAGTAC | 140 | ||
| R | GTCACCTATGGCTGCAATGCT | ||||
| Apoptosis, hypertrophy, and fibrosis markers | NM_006172.4 | F | CAGGATGGACAGGATTGGA | 79 | |
| R | TGTCCTCCCTGGCTGTTATC | ||||
| NM_002521.3 | F | TTGGAAACGTCCGGGTTAC | 126 | ||
| R | GGACTTCCAGACACCTGTGG | ||||
| NM_001844 | F | GGCAATAGCAGGTTCACGTACA | 79 | ||
| R | CGATAACAGTCTTGCCCCACTTA | ||||
| NM_138761.4 | F | TTCCGACGGCAACTTCAACT | 204 | ||
| R | GGTGACCCAAAGTCGGAGAG | ||||
| NM_000633.2 | F | TCATGTGTGTGGAGAGCGTCAA | 179 | ||
| R | CAGCCAGGAGAAATCAAACAGAGG | ||||
| Housekeeping gene | NM_002046.5 | F | ACCCACTCCTCCACCTTTGA | 101 |