Fig. 2. CM differentiation in iPSCs derived from healthy controls and patients with SLE. (a) Schematic diagram of the procedures used to differentiate iPSCs into CMs in 2D culture. (b) CM morphology on days 0, 2, 4, and 10 of differentiation. (c) CM marker gene expression levels in HC and SLE iPSCs, HC-CM and SLE-CM were evaluated by RT-PCR (n=3 per group). (d) CM marker protein level in iPSC and CM of HC and SLE. (e) Beating of CMs generated from HC-iPSCs and SLE-iPSCs. (f) Expression levels of cardiac-specific markers in iPSC-derived CMs were assessed by immunofluorescence staining and confocal microscopy. HC-CMs and SLE-CMs showing characteristic sarcomeric structures and positive staining with anti-TNNT2 antibody and DAPI for nucleic acid. (g) Fluorescence recording of calcium flux from HC-CMs and SLE-CMs. (h) Sarcomeric structures in HC-CMs and SLE-CMs depicted by high-magnification transmission electron microscopy (HM-TEM). Scale bar indicates 200 μm.

International Journal of Stem Cells 2022;15:233-46 https://doi.org/10.15283/ijsc21158

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