International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. Comparison of 2D-CM and 3D-CM differentiation in HC-iPSCs. (a) Schematic diagram of procedures used to differentiate iPSCs into CMs on a 3D culture platform. (b) CM morphology at days 0, 2, 4, and 10 of differentiation. (c) Expression levels of early cardiac marker NKX2-5 and cardiomyocyte markers TNNT2 and MYH6 measured in HC-2D and 3D-CMs (n=3 per group) by RT-PCR. (d) Representative image of whole staining of TNNT2 in CM-3D. (e) Representative images of EB (negative control) and CM-3D stained with TNNT2. Scale bar indicates 200 μm. (f) Calcium signals were also identified in the final 3D-CMs.
International Journal of Stem Cells 2022;15:233-46
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