International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Autoantibody-blocking culture system of HC-CMs in active SLE serum. (a) Schematic diagram of autoantibody-blocking culture system of HC-CMs in active SLE serum. (b) Morphology of 3D-CMs subjected to autoantibody treatment for 2 d followed by SLE-activated serum for 2 d. Scale bar=100 μm. (c) Beating rates in each group. (d) Cell viability assay of autoantibody-blocking culture system. Scale bar=100 μm. (e) Graph of cell viability assay. (f) Graph of calcium signal rhythm based on calcium functional staining. (g) Expression levels of apoptosis (BAX/Bcl2 ratio), fibrosis (COL2A1), and hypertrophy (ANP, BNP) markers in autoantibody-blocking culture system were quantified by RT-PCR. The sample number of each group was n=3 for gene expression confirmation. (h) Representative image of confirmed caspase 3 and 8 proteins in the 3D-CMs. Scale bar indicates 200 μm.
International Journal of Stem Cells 2022;15:233-46 https://doi.org/10.15283/ijsc21158
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