International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. Diesel exhaust particles (DEP) affect WJ-MSC function. (a, b) The WJ-MSCs were treated with DEP (0∼40 μg/ml) for 48 hr. (a) Cell viability was assessed using an MTT assay. (b) Early and late apoptosis were measured by labeling with Annexin-V-FITC and 7-AAD for flow cytometry analysis. (c) A scratched wound healing assay of DEP-treated WJ-MSCs (0∼10 μg/ml) was performed to analyze cell migration into the scratched area. After 18 and 36 hr DEP treatment, migration into the scratched area was measured. (Scale bar: 200 μm). (d, e) DEP-treated WJ-MSCs were cultured with osteogenic differentiation medium for 21 days. (d) Representative light microscopic images of osteocytes. Calcium deposits in cells cultured under osteogenesis were stained with Alizarin Red S (scale bar: 100 μm) and quantified by measuring absorbance using a spectrophotometer. Blue arrows indicate DEP. (e) Expression of an osteogenic gene (RUNX2) was quantified by qRT-PCR with β-actin as the reference gene. Data are presented as the mean±S.D. of triplicate experiments (*p<0.05; **p<0.01; ***p<0.001).
International Journal of Stem Cells 2022;15:203-16
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