International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. DEP-treated WJ-MSCs express ROS and pro-inflammatory genes. (a) Heatmap of hierarchical clustering. Differentially expressed gene (DEG) profile for the control and DEP-treated WJ-MSCs were analyzed (p-value<0.05). (b) Volcano plot highlighting DEGs for the control and DEP-treated WJ-MSCs and notable genes of interest. The blue dots represent significantly upregulated genes, and the red dots represent significantly downregulated genes (|log2 FC|≥1 and FDR<0.01). (c) Transcription of cFos was quantified by qRT-PCR. (d) The WJ-MSCs were incubated with DEP at the indicated concentrations for 6 hr. Intracellular ROS levels were measured using the ROS-sensitive fluorophore 2’,7’-dichlorofluorescin diacetate (DCFDA) and analyzed by flow cytometry. (e) Transcription of ROS-related genes was determined by qRT-PCR and normalized to β-actin expression. (f) Transcription of inflammation-related genes was determined by qRT-PCR and normalized to β-actin expression. The data are presented as the mean±S.D. of three independent experiments (*p<0.05; **p<0.01; ***p<0.001).
International Journal of Stem Cells 2022;15:203-16 https://doi.org/10.15283/ijsc21178
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