International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

Download original image
Fig. 3. DEP-induced ROS inhibits the immunomodulatory effects of WJ-MSCs. (a∼c) WJ-MSCs were pretreated for 30 min with NAC (5 mM) and then were mitotically inactivated after treating with various concentrations of DEP for 48 hr. Cells were co-cultured with CFSE-labeled human mononuclear cells (MNCs) that were activated by CD3/CD28 Dynabeads and IL-2. After six days of co-culture, the percentage of T cell proliferation was measured using flow cytometry. (a) Representative histogram of total lymphocyte proliferation. (b) Proliferation of total, CD4+ T, and CD8+ T lymphocytes was quantified. (c) The number of cell divisions of total, CD4+ T, and CD8+ T lymphocytes was quantified based on dilution of CFSE. (d, e) Cells were pretreated for 30 min with NAC and then were stimulated with 10 μg/ml DEP for 24 hr. (d) cFos mRNA was determined by qRT-PCR and normalized to β-actin mRNA. (e) cFos expression was quantified by western blot analysis. The data represent results from three independent experiments and are presented as mean±SD (*p<0.05; **p<0.01; ***p<0.001).
International Journal of Stem Cells 2022;15:203-16 https://doi.org/10.15283/ijsc21178
© 2021 International Journal of Stem Cells