International Journal of Stem Cells : eISSN 2005-5447

Fig. 1.

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Fig. 1. Abnormal blood vessel development in P14 cerebral cortex and hippocampal dentate gyrus of MNK mice. (A) GLUT1 (marker of brain microvessel) was used to analyze brain vessel pattern in cerebral cortex of wild type and MNK mice. Expression of GLUT1 was decreased in P14 cerebral cortex of MNK mice compared with that of wild type. Scale bar 100 μm (upper), 50 μm (lower). Arrowheads in high magnification image show that sprouting formation in neovascular endothelial tip was reduced in P14 cerebral cortex of MNK mouse. DAPI was used to counterstain nuclei (Blue). (B, C) Quantitative data of blood vessels length show that number and proportion of blood vessels less than 25 μm length was increased in the P13 MNK cerebral cortex. *p<0.05, **p<0.01 by t-test. (D) Reduction of brain vessels revealed by microCT scan indicates the defect of cerebral vascular structure in the P14 of MNK mice. The red box indicates regions shown in below. (E) P13 hippocampal dentate gyrus of MNK mice were stained with neural stem cell marker NESTIN and cerebral vessel marker GLUT1. Decreased NESTIN+ radial processes and blood vessels in the P13 hippocampal dentate gyrus of MNK mice were observed compared to wild type littermate. Scale bar 50 μm. SGZ, subgranular zone; GCL, granule cell layer; ML, molecular layer.
International Journal of Stem Cells 2022;15:270-82 https://doi.org/10.15283/ijsc21088
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