Fig. 5. Inhibition of RA signaling pathway by BMS biases the differentiation of hiPSC to Sinoatrial node-like cells. BMS at 5 μM was introduced on day 5 after the differentiation, and the expression of COUPTFII (A, B), TBX18 (C, D), and TBX3 (E, F) was evaluated by IF. Representative images and corresponding quantitations showing that inhibition of RA signaling pathway by BMS decreases the percentage of COUPTFII (A, B) positive cardio-myocytes and increases the percentage of TBX18-(C, D) and TBX3-positive (E, F) cardiomyocytes. Scale bars=100 μm (400× magnification).

International Journal of Stem Cells 2022;15:247-57 https://doi.org/10.15283/ijsc21148

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