International Journal of Stem Cells : eISSN 2005-5447

Fig. 7.

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Fig. 7. Inhibition of RA signaling pathway by BMS biases the differentiation of cardiomyocytes possessing typical electrophysiological features of Sinoatrial node-like cells. BMS at 5 μM was introduced on day 5 after the differentiation, and the beating frequency and AP were analyzed after the differentiation on day 21 and 60 respectively. BMS significantly increased the beating frequency of cardiomyocytes (A), sensitized cardiomyocytes to TCN ion channel inhibitor (B), decreased the ratio of cardiomyocytes displaying typical atrial-like AP (C, middle panel; D, blue bar, 5/15), and increased the ratio of cardiomyocytes displaying typical pacemaker-like AP (C, bottom panel; D, green bar, 4/15), compared to RA group (10/15 and 2/15 respectively). Data in (A) and (B) are presented as ‘Mean±SD’ from recording of 7 cardiomyocytes, with *denoting p<0.05 and ****denoting p<0.0001.
International Journal of Stem Cells 2022;15:247-57 https://doi.org/10.15283/ijsc21148
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