Fig. 1. Morphology, phenotypic, and multidirectional differentiation characteristics of mouse BMSCs. (A) Re-presentative morphology image of passage 2 (P2) BMSCs was visual-ized by light microscopy (Scale bar, 100 μm). (B) P3 BMSCs surface markers were identified by flow cyto-metry. (C) Osteogenic differentiation capacities of isolated BMSCs were verified by alizarin red staining after incubation for 14 days (n=3; Scale bar, 100 μm). (D) Adipogenic differentiation capacities of isolated BMSCs were detected by oil red O staining after culture for 14 days (n=3; Scale bar, 100 μm). BMSCs: bone mesenchymal stem cells.
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