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Sequences of primers used for qRT-PCR
| Gene | Forward primer sequence (5’–3’) | Reverse primer sequence (5’–3’) |
|---|---|---|
| β-actin | CTACCTCATGAAGATCCTGACC | CACAGCTTCTCTTTGATGTCAC |
| TNF-α | ATGTCTCAGCCTCTTCTCATTC | GCTTGTCACTCGAATTTTGAGA |
| IL-6 | TCTGGAGCCCACCAAGAACGATAG | GTCACCAGCATCAGTCCCAAGAAG |
| Runx2 | CCTTCAAGGTTGTAGCCCTC | GGAGTAGTTCTCATCATTCCCG |
| ALP | AACCCAGACACAAGCATTCC | CCAGCAAGAAGAAGCCTTTG |
| OCN | GGACCATCTTTCTGCTCACTCTGC | TCCTGCTTGGACATGAAGGCTTT |
qRT-PCR: quantitative real-time polymerase chain reaction, TNF-α: tumor necrosis factor-α, IL-6: interleukin-6, Runx2: runt-related transcription factor 2, ALP: alkaline phosphatase, OCN: osteocalcin.