Fig. 2. CBD inhibits LPS-induced inflammatory response. (A) BMSCs’ viability was tested by CCK-8 assay after incubating with different concentrations of CBD for 72 hrs (n=8). (B) BMSCs were pretreated with LPS (10 μg/ml) for 12 hrs, and added different concentrations of CBD (0.5, 2.5, 5 μM) for 12 hrs. Then, BMSCs were harvested for detecting the mRNA expression levels of TNF-α and IL-6. β-actin was used as the internal control (n=5). CBD: cannabidiol, LPS: lipopolysaccharide, BMSCs: bone mesenchymal stem cells, CCK-8: cell counting kit-8, TNF-α: tumor necrosis factor-α, IL-6: interleukin-6, ns: no statistical significance. Data are presented as means±SD. *p<0.05 compared with the LPS group; ^#p<0.05 compared with the LPS plus 0.5 μM CBD group.

International Journal of Stem Cells 2022;15:405-14 https://doi.org/10.15283/ijsc21152

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