International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Quantitative analysis of EGF secretion and effect of hUCB-MSCs on migration of HaCaT cells. (A) Measurement of EGF secretion according to density of hUCB-MSCs (n=4). (B) Measurement of EGF secretion according to culture duration of hUCB-MSCs (n=4). (C) HaCaT cells, HDFs, and HEK293FT cells secrete low levels of EGF, whereas hUCB-MSCs secrete a high level of EGF (n=4). (D) HaCaT cells were cultured until they reached 90% confluency and then scratched with a sterile pipette tip. Representative microscopic images of scratched cells after co-culture with hUCB-MSCs for 48 h are shown. (E)The length of the wound was expressed as mean±SEM of three independent experiments. Cont: positive control, MSC: mesenchymal stem cells, EGF: epidermal growth factor. *p<0.05, **p<0.01, and ***p<0.001.
International Journal of Stem Cells 2022;15:311-23 https://doi.org/10.15283/ijsc21173
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