Fig. 1. Percentage of active MSCs in muscle-derived primary cell populations isolated using different muscle dissociation protocols. Each primary cell population was derived from skeletal muscle tissues of 3-week-old mice according to the experimental design described in Fig. S1. Muscle-derived primary cell populations were double-stained with antibodies detecting Pax7 (red) and MyoD (green), which are expressed simultaneously in active MSCs. The percentage of double-stained cells was determined by multiplying the number of double-stained cells divided by the total number of cells with 100. MSCs that exhibit positive double-staining for Pax7 and MyoD were observed within muscle-derived primary cell populations, irrespective of the muscle dissociation protocol (A). In all experimental replicates, primary cell populations obtained using Method 3 exhibited the highest percentage of active MSCs (B). Representative images of the co-expression of MyoD and Pax7 in active MSCs are displayed. Nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole (DAPI; blue), n=5. Scale bars represent 50 μm. All data are presented as the mean (dotted line) of five independent experiments.

International Journal of Stem Cells 2022;15:283-90 https://doi.org/10.15283/ijsc21179

© 2022 International Journal of Stem Cells