International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Effects of DP method on improving active MSC purity from muscle-derived primary cell populations. The DP method was used to retrieve putative MSCs from muscle-derived primary cell populations of 3-week-old mice generated using Method 3. The cells were double-stained with antibodies against Pax7 (red) and MyoD (green), which are expressed simultaneously in active MSCs, and nuclear counterstaining was performed using DAPI (blue). Co-localization of Pax7 and MyoD in the nuclear region indicates active MSCs (A). The percentage of double-stained cells (indicating active MSCs) was determined by multiplying the number of double-stained cells divided by the total number of cells with 100. The percentage of active MSCs co-expressing Pax7 and MyoD was significantly higher in populations subjected to the DP method (B). All figures shown in (A) are representative images of the co-expression of Pax7 and MyoD in active MSCs, and all data shown in (B) represent the mean±SD of three independent experiments. *p<0.05, n=3 in (A). Scale bars represent 50 μm.
International Journal of Stem Cells 2022;15:283-90
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