International Journal of Stem Cells : eISSN 2005-5447

Fig. 3.

Download original image
Fig. 3. Effects of difference in mouse age on isolating active MSCs from skeletal muscle tissues. Muscle-derived primary cell populations were retrieved from skeletal muscle tissues from 1-, 2-, 3-, and 4-week-old mice using Method 3. Collection of putative MSCs from each muscle-derived primary cell population was conducted using the DP method. All putative MSCs were double-stained with anti-Pax7 and anti-MyoD antibodies. Total cell numbers and the numbers of double-stained cells were counted. In all repeated experiments, the highest number of cells double-stained for Pax7 and MyoD was consistently observed in putative MSCs derived from 2-week-old mice compared to those from 1-, 3-, and 4-week-old mice. All data are presented as the mean (dotted line) of three independent experiments. *p<0.05.
International Journal of Stem Cells 2022;15:283-90
© 2022 International Journal of Stem Cells