Fig. 4. Vascularization of hCOs with vascular spheroids reduces apoptosis. (A) Representative images of the TUNEL assay for detecting apoptotic cells. Quantification showing the percentage of TUNEL＋ cells that were positive for DAPI in each group (Scale bar: 100 μm). (B) Protein levels of rH2AX and cleaved caspase-3. (C) Immunostaining images and quantification of rH2AX and SOX2 in each group on day 60 (Scale bar: 100 μm). (D) Immunostaining images and quantification of cleaved caspase-3 and PAX6 in each group on day 60 (Scale bar: 100 μm). Student’s t-test, n=4, independent organoids from 2 different batches. *p<0.05, ***p<0.001; n.s. not significant.

International Journal of Stem Cells 2022;15:85-94 https://doi.org/10.15283/ijsc21157

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