International Journal of Stem Cells : eISSN 2005-5447

Fig. 5.

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Fig. 5. Wnt/β-catenin signaling are upregulated in ahCOs. (A) Western blot of Wnt7a, β-catenin, GSK3β, AXIN2 and GFAP in each group of organoids. Western blot analysis revealed that decrease tendency of GSK3β and up-regulation of axin2 along with Wnt7a activation and that β-catenin was increased in the ahCO group compared to the hCO group. (B) Immunostaining images and quantification of SOX2 and NeuN in each group on day 60 (Scale bar: 100 μm). (C) Immunostaining images and quantification of MAP2 and GFAP in each group on day 60 (Scale bar: 100 μm). Student’s t-test, n=4, independent organoids from 2 different batches. **p<0.01; n.s. not significant.
International Journal of Stem Cells 2022;15:85-94 https://doi.org/10.15283/ijsc21157
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