Fig. 1. Characterization of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). (A) Phase contrast images of hESCs and hiPSCs in feeder-free conditions. Scale bar=200 μm. (B) Immunocytochemical staining of the pluripotency markers OCT4, SOX2, and TRA-1-81 in hESCs (upper) and hiPSCs (lower). Scale bar=200 μm in the upper panels and 100 μm in the lower panels. (C) Growth rate of hESCs from 2 to 4 days after seeding. Growth rate was measured as an increase in colony size (μm2). Error bars represent standard error of the mean.
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