International Journal of Stem Cells : eISSN 2005-5447

Fig. 2.

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Fig. 2. Brain organoid differentiation protocol using human pluripotent stem cells (hPSCs). (A) Schematic illustration of the generation of brain organoids. Representative images characterizing each stage are shown. Scale bar=200 μm. (B) Morphology of embryoid bodies (EBs) in EB formation medium, based on TeSR medium (upper left), and their enlarged images (upper right). EBs committed to the neural lineage showed a transparent neuroepithelium-like region at the periphery of the structure (lower left), which is depicted with a white dotted circle (lower right). Scale bar=500 μm (lower left), 200 μm (lower right). (C) Comparison of koSR-containing medium (hESC medium) and Aggrewell EB medium for the size of EBs differentiated from hESCs. Scale bars=200 μm.
International Journal of Stem Cells 2022;15:95-103
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