International Journal of Stem Cells : eISSN 2005-5447

Fig. 4.

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Fig. 4. Characterization of human embryonic stem cell (hESC)- and human induced pluripotent stem cell (hiPSC)-derived brain organoids. (A) 2D immunocytochemistry staining of the early neuron marker TUJ-1 and the neural progenitor marker SOX2 in hPSC-derived organoids. A Cryostat (upper) and Vibratome (lower) were used for tissue sectioning. Scale bar=100 μm (upper panels) and 200 μm (lower panels). Green arrow indicates cortical plate, red arrow indicates ventricular zone, and white arrow indicates ventricle (Cryostat sample). Green arrow indicates ventricular zone, red arrow indicates cortical plate, and white arrow indicates ventricle (Vibratome sample). (B) Confocal images of 3D immunocytochemistry for the early neuron marker TUJ-1 and the neural progenitor marker SOX2 in hESC- and hiPSC-derived organoids. Scale bar=50 μm (upper panels) and 20 μm (lower panels). (C) Representative 3D image of whole brain organoids, which were stained with TUJ-1 and SOX2 (at day 12 hESC-derived). The image was captured using high contents screening (HCS). Scale bars=200 μm.
International Journal of Stem Cells 2022;15:95-103 https://doi.org/10.15283/ijsc21195
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