Fig. 1. Culture and characterization of InS^exp derived from 3D HIOs. (A) Schematic diagram of the InS^exp culture method. (B, C) Bright field images of InS^exp by data, after thawing (B), and by passaging (C). White scale bars, 500 μm. Yellow scale bars, 250 μm. (D) Relative expression of intestinal epithelial cell marker genes in hPSCs, InS^exp, and HIOs. (E) Immunofluorescence analysis of intestinal epithelial cell markers in InS^exp. White scale bars, 50 μm. (F) Bright field images of 3D HIOs, InS^exp, and re-embedded InS^exp. White scale bars, 500 μm. Yellow scale bars, 250 μm. *p<0.05, **p<0.01, ***p<0.001 using two-tailed t-test.

International Journal of Stem Cells 2022;15:104-11 https://doi.org/10.15283/ijsc21209

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