Fig. 2. CHIR99021 treatment promotes growth rate of InS^exp. (A, B) Effect of CHIR99021 on the InS^exp growth rate at multiple concentrations. Bright field images of the InS^exp (A) and Relative fold change in the InS^exp cell number (B). White scale bars, 500 μm. Data represent means±SEM (n=4). (C, D) Recovery of InS^exp-forming competency by addition of CHIR99021 in WNT3A- and R-spondin 1-depleted medium. Bright field images of InS^exp (C) and Relative fold change in the InS^exp cell number (D). White scale bars, 500 μm. Yellow scale bars, 250 μm. Data represent means±SEM (n=4). (E) Inhibition of InS^exp growth by treatment with WNT inhibitors. White scale bars, 500 μm. Data represent means±SEM (n=4). (F) Immunofluorescence analysis and quantification of proliferating cell markers in InS^exp. Scale bars, 200 μm. Data represent means±SEM (n=5). *p<0.05, **p<0.01, ***p<0.001 using two-tailed t-test.

International Journal of Stem Cells 2022;15:104-11 https://doi.org/10.15283/ijsc21209

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